KMID : 0383820120730040204
|
|
Tuberculosis and Respiratory Diseases 2012 Volume.73 No. 4 p.204 ~ p.209
|
|
Effect of Chrysin on Gene Expression and Production of MUC5AC Mucin from Cultured Airway Epithelial Cells
|
|
Shin Hyun-Dae
Lee Hyun-Jae Md. Asaduzzaman Sikder Park Su-Hyun Ryu Ji-Ho Hong Jang-Hee Kim Ju-Ock Seok Jeong-Ho Lee Choong-Jae
|
|
Abstract
|
|
|
Background: We investigated whether chrysin affected MUC5AC mucin production and gene expression induced by phorbol ester (phorbol 12-myristate 13-acetate, PMA) or epidermal growth factor (EGF) from human airway epithelial cells.
Materials and Methods: Confluent NCI-H292 cells were pretreated with varying concentrations of chrysin for 30 minutes, and were then stimulated with PMA and EGF for 24 hours, respectively. MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay.
Results: Concentrations of 10¥ìM and 100¥ìM chrysin were found to inhibit the production of MUC5AC mucin protein induced by PMA; A concentration of 100¥ìM chrysin also inhibited the production of MUC5AC mucin protein induced by EGF; 100¥ìM chrysin inhibited the expression of MUC5AC mucin gene induced by PMA or EGF. The cytotoxicity of chrysin was checked by lactate dehydrogenase assay, and there was no cytotoxic effect observed for chrysin.
Conclusion: These results suggest that chrysin can inhibit mucin gene expression and the production of mucin protein by directly acting on airway epithelial cells.
|
|
KEYWORD
|
|
Epithelial Cells, Mucins, Chrysin
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|